Natural Resources Institute Finland (Luke) 2017 Efficacy evaluation of fungicides against net blotch in spring barley - Advisory fungicide trial

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1 2017 Efficacy evaluation of fungicides against net blotch in spring barley - Advisory fungicide trial SUMMARY The trial was located in Jokioinen in Kuuma. The cultivar in the trial was Voitto which is quite susceptible to net blotch. Previous crop was oat. The barley was drilled on 29 th of May. Weather during the season differed from the long term average summer. May was dry until the end of the month when there were the first rainy days. May was also quite cold even before sowing the trials there was a warm period. In June it rained 101 mm which is almost 40 mm over the long term average. The mean temperature was 12.8 C which is also lower to the 14 C average. In July it rained 44.7 mm which is 30 mm below the average, but the mean temperature was 14.9 C which is 1.8 C below the average. It meant that the soil and also crop stayed wet for long periods. August was again a bit rainier and colder than the long term average. September was then quite normal and it rained a bit less. The effective temperature sum stayed whole summer C below the long term average. At the end of September the temperature sum was 1136 while the average is The beginning of the growth was really slow and barley also suffered from too wet soil because of some heavy rains. After that crop stand was good and dense. All fungicide treatments were done on 7 th of July. The growth stage was BBCH 37 and flag leaves were visible, but not yet open. At application time there was net blotch (Pyrenophora teres) only around 3 % in the fourth leaf. The first disease assessment was done two weeks after the treatment at BBCH 69. The net blotch severity was 11 % in the untreated control and the severity was significantly lower with all treatments, around 1 2 %. There was no phytotoxicity with any of the treatments. Four weeks after treatments net blotch development was fast, because the conditions were favorable. Disease severity in the untreated control was already 86 %. In Proline treatment net blotch severity was 14 % and Orius+Mirador 11 %, but with all other treatments severity was only %. Elatus Era and Ascra Xpro treatments had slightly lower disease severity, but also Comet Pro+Juventus, Elatus Plus+Amistar Xtra, Comet Pro+Prosaro and Delaro had good efficacy. Green leaf area at milk ripening stage in the untreated control was only 7.5 % on the flag leaf. In all treated plots four leaf layers were still partly green and green leaf area was %. The trial was harvested on 27 th of September. The yield of untreated control was 3800 kg/ha. The yield increase with all treatments was % to kg/ha, but there was no statistical difference between the treatments. Highest yield was achieved with Elatus Era and Ascra Xpro, over 5500 kg/ha. Those treatments had also the highest efficacy against diseases. Hectoliter weight in the untreated control was 64.8 kg, but all treatments increased it 6 9 % to kg. Again Elatus Era and Ascra Xpro had the highest increase in HLW. Thousand grain weight was 34.3 g in the untreated control. Thousand grain weight with all treatments was % higher, g. With Orius+Mirador, Delaro and Proline increase in TGW was %, when it was % with Comet Pro+Prosaro, Elatus Plus+Amistar Xtra, Comet Pro+Juventus, Ascra Xpro and Elatus Era. The reason for higher yield, HLW and TGW was that with fungicide treatments the crop stayed green three to four weeks longer than in the untreated control.

2 2017 Efficacy evaluation of fungicides against net blotch in spring barley - Advisory fungicide trial TIIVISTELMÄ Markkinoilla olevia viljojen sienitautien torjunta-aineita testattiin ohralla kenttäkokeessa Jokioisissa. Testatut valmisteet olivat Proline 0.6 l/ha, Comet Pro + Juventus l/ha, Orius + Mirador l/ha, Elatus Era 1.0 l/ha, ElatusPlus + Amustar Xtra l/ha, Comet Pro + Prosaro l/ha, Ascra Xpro 1.0 l/ha ja Delaro 0.7 l/ha. Kokeeseen sisältyi myös käsittelemätön koejäsen. Koe suunniteltiin yhteistyössä kasvinsuojeluaineyritysten kanssa ja toteutettiin GEP-laatujärjestelmän mukaisesti. Kasvukauden sää oli tavanomaisesta vähän poikkeava. Toukokuussa oli kuivaa ja vasta kuun lopussa tuli enemmän sateita, mutta sademäärä oli vain 13 mm ja alle pitkän ajan keskiarvon joka on 41mm. Toukokuu oli viileä lukuun ottamatta yhtä lämmintä jaksoa. Keskilämpötila oli silti asteen normaalia 9.8 C alhaisempi. Kesäkuussa vettä satoi 101 mm ja se on 40 mm yli pitkäaikaisen keskiarvon. Keskilämpötila oli 12.8 C, kun se normaalisti on 14 C. Heinäkuussa satoi 45 mm eli 30 mm tavallista vähemmän. Myös keskilämpötila oli 14.9 C ollen 1.8 C alle tavanomaisen. Ilmankosteus oli korkea koko kuukauden ja maa ja kasvusto eivät päässeet kuivumaan. Elokuu oli pitkän ajan keskiarvoa vähän viileämpi ja kosteampi. Kesä-, heinä ja elokuussa päivän maksimilämpötilat olivat harvemmin yli 20 C ja hellepäiviä ei ollut. Syyskuussa oli poutaisempaa ja vähän lämpöisempää, joten viljat pääsivät tuleentumaan. Ohrakoe sijaitsi Jokioisilla Kuumassa ja se kylvettiin Lajikkeena oli Voitto, mikä on melko verkkolaikun altis lajike. Esikasvina oli kaura. Kasvuunlähtö oli hidasta ja ohra kärsi liian märästä maasta, sillä kesäkuussa oli sateista ja maa ei ehtinyt kuivumaan. Kesäkuun loppupuolella ohra alkoi kasvaa kunnolla ja kasvustosta tuli oikein rehevä. Käsittely tehtiin 7.7. lippulehtivaiheessa BBCH 37, kun lippulehdet eivät olleet vielä täysin auki. Käsittelyhetkellä verkkolaikkua oli neljännellä lehdellä noin 3 %. Kaksi viikkoa ruiskutuksen jälkeen ohra oli kukinnan loppuvaiheessa. Käsittelyillä ei ollut vioituksia ohralla. Käsittelemättömässä kontrollissa verkkolaikkua oli 11 % lehtialasta, mutta kaikilla käsittelyillä tautimäärä oli vain 1 2 %. Käsittelyiden välillä ei ollut vielä eroa. Verkkolaikku levisi seuraavan kahden viikon aikana runsaasti ja maitotuleentumisvaiheessa käsittelemättömissä ruuduissa taudin määrä oli jo 86 %. Proline ja Orius+Mirador käsittelyjen teho oli heikentynyt, mutta oli yhä % (verkkolaikkua %). Muiden käsittelyjen teho oli yhä %, verkkolaikkua oli siis vain 1 4 % lehtialasta. Elatus Eran ja Ascra Xpron teho oli hieman muita käsittelyjä parempi. Vihreä lehtiala oli käsittelemättömissä ruuduissa enää noin 8 %, kun taas käsittelyillä % lehtialasta oli vielä vihreää. Parhailla käsittelyillä jopa neljä ylintä lehteä oli vielä vihreitä ja säilyivät taudista puhtaina kellastumiseen asti. Koe puitiin syyskuun lopussa. Käsittelemättömän kontrollin sato oli 3800 kg/ha. Kaikilla käsittelyillä oli selvä % sadonlisäys ja sato oli kg/ha. Käsittelyjen välillä ei ollut tilastollisesti merkitsevää eroa. Parhaat sadot tuottivat Elatus Era ja Ascra Xpro käsittelyt, jotka pitivät kasvuston myös vihreimpänä pisimpään. Hehtolitrapaino oli ilman käsittelyä 64.8kg ja käsittelyt lisäsivät sitä 6 9%, jolloin se oli kg. Tuhannen jyvän paino oli käsittelemättömässä kontrollissa 34.3 g. Myös TJP lisääntyi eri käsittelyillä % ollen siten %. Parhaat käsittelyt sadon määrässä ja laadussa olivat Elatus Era ja Ascra Xpro, mutta hyvin lähellä olivat myös Elatus Plus+Amistar Xtra ja Comet Pro+Juventus.

3 2017 Efficacy evaluation of fungicides against net blotch in spring barley - Advisory fungicide trial FIGURES Figure 1. Net blotch severity was rising in the untreated plots two weeks after the fungicide treatments.. Figure 2. One month after treatments most treatments had really good control compared to the untreated control.

4 2017 Efficacy evaluation of fungicides against net blotch in spring barley - Advisory fungicide trial Figure 3. Green leaf area at late milk ripening stage was still high. Figure 4. The yield increase with all treatments was %. The yield differences between the treatments were not statistically significant in this trial.

5 2017 Efficacy evaluation of fungicides against net blotch in spring barley - Advisory fungicide trial Figure 5. The increase in hectoliter weight was 6 9 % with all the treatments. Figure 6. Higher yield was due to % higher thousand grain weight. There were also differencens between the treatments.

6 18/01/18 (F ) ARM Trial Treatments Page 1 of 1 Efficacy evaluation of fungicides against net blotch in spring barley Trial ID: F Location: Jokioinen Trial Year: 2017 Protocol ID: F Investigator: Kalle Ohralahti Project ID: Advisory Trials Study Director: Kalle Ohralahti Sponsor Contact: Luke Trt Treatment Form Form Rate Growth Appl Comment No. Name Conc Type Rate Unit Stage Code 1 1 Untreated Check 2 Proline 250 EC 0.6 l/ha 37 A Luke -prothioconazole g AI 3 Comet Pro 200 EC 0.4 l/ha 37 A Luke -pyraclostrobin g AI Juventus 90 SL 0.4 l/ha 37 A Luke -metconazole g AI 4 Orius 200 EW 0.8 l/ha 37 A Adama -tebuconazole g AI Mirador 250 SC 0.4 l/ha 37 A Adama -azoxystrobin g AI 5 ElatusEra 225 EC 1 l/ha 37 A Syngenta -solatenol g AI -prothioconazole g AI 6 ElatusPlus 100 EC 0.5 l/ha 37 A Syngenta -solatenol g AI Amistar Xtra 280 SC 0.5 l/ha 37 A Syngenta -azoxystrobin g AI -cyproconazole g AI 7 Comet Pro 200 EC 0.4 l/ha 37 A Bayer -pyraclostrobin g AI Prosaro 250 EC 0.4 l/ha 37 A Bayer -tebuconazole g AI -prothioconazole g AI 8 Ascra Xpro 260 EC 1 l/ha 37 A Bayer -bixafen g AI -fluopyram g AI -prothioconazole g AI 9 Delaro 325 FS 0.7 l/ha 37 A Bayer -trifloxystrobin g AI -prothioconazole g AI Replications: 4, Untreated treatments: 1, Conduct under GLP/GEP: Yes (GEP with no protection), Design: Randomized Complete Block (RCB), Treatment units: Treated 'Plot' experimental unit size, Dry Form. Unit: %, Treated 'Plot' experimental unit size Width: 2 meters, Treated 'Plot' experimental unit size Length: 8 meters, Application volume: 200 L/ha, Mix size: 2.5 liters, Format definitions: G-All7.def, G-All7.frm

7 18/01/18 (F ) ARM Site Description Page 1 of 4 Efficacy evaluation of fungicides against net blotch in spring barley Trial ID: F Location: Jokioinen Trial Year: 2017 Protocol ID: F Investigator: Kalle Ohralahti Project ID: Advisory Trials Study Director: Kalle Ohralahti Sponsor Contact: Luke General Trial Information Study Director: Kalle Ohralahti Title: Research Scientist Investigator: Kalle Ohralahti Title: Research Scientist Discipline: F fungicide Trial Status: F final (completed) Trial Reliability: GOOD Initiation Date: 29/05/17 Completion Date: 15/11/17 Trial Location City: Jokioinen Country: FIN South Ostrobothnia State/Prov.: Kuuma Postal Code: Climate Zone: EPPONE EPPO North East Latitude of LL Corner : Longitude of LL Corner : Angle y-axis to North : N E Conducted Under GLP: No Conducted Under GEP: Yes No. Guideline Description 1. PP 1/26(4) Foliar and ear diseases on cereals 2. PP 1/135(4) phytotoxicity assessment 3. PP 1/152(4) Design and analysis of efficacy evaluation trials 4. PP 1/181(4) Conduct and reporting of efficacy evaluation trials including GEP Objectives: The efficacy of fungicides on market against net blotch in spring barley.

8 18/01/18 (F ) ARM Site Description Page 2 of 4 Conclusions: The trial was located in Jokioinen in Kuuma. The cultivar in the trial was Voitto which is quite susceptible to net blotch. Previous crop was oat. The barley was drilled on 29th of May. Weather during the season differed from the long term average summer. May was dry until the end of the month when there were the first rainy days. May was also quite cold even before sowing the trials there was a warm period. In June it rained 101 mm which is almost 40 mm over the long term average. The mean temperature was 12.8 C which is also lower to the 14 C average. In July it rained 44.7 mm which is 30 mm below the average, but the mean temperature was 14.9 C which is 1.8 C below the average. It meant that the soil and also crop stayed wet for long periods. August was again a bit rainier and colder than the long term average. September was then quite normal and it rained a bit less. The effective temperature sum stayed whole summer C below the long term average. At the end of September the temperature sum was 1136 while the average is The beginning of the growth was really slow and barley also suffered from too wet soil because of some heavy rains. After that crop stand was good and dense. All fungicide treatments were done on 7th of July. The growth stage was BBCH 37 and flag leaves were visible, but not yet open. At application time there was net blotch (Pyrenophora teres) only around 3 % in the fourth leaf. The first disease assessment was done two weeks after the treatment at BBCH 69. The net blotch severity was 11 % in the untreated control and the severity was significantly lower with all treatments, around 1-2 %. There was no phytotoxicity with any of the treatments. Four weeks after treatments net blotch development was fast, because the conditions were favorable. Disease severity in the untreated control was already 86 %. In Proline treatment net blotch severity was 14 % and Orius+Mirador 11 %, but with all other treatments severity was only %. Elatus Era and Ascra Xpro treatments had slightly lower disease severity, but also Comet Pro+Juventus, Elatus Plus+Amistar Xtra, Comet Pro+Prosaro and Delaro had good efficacy. Green leaf area at milk ripening stage in the untreated control was only 7.5 % on the flag leaf. In all treated plots four leaf layers were still partly green and green leaf area was %. The trial was harvested on 27th of September. The yield of untreated control was 3800 kg/ha. The yield increase with all treatments was % to kg/ha, but there was no statistical difference between the treatments. Highest yield was achieved with Elatus Era and Ascra Xpro, over 5500 kg/ha. Those treatments had also the highest efficacy against diseases. Hectoliter weight in the untreated control was 64.8 kg, but all treatments increased it 6-9 % to kg. Again Elatus Era and Ascra Xpro had the highest increase in HLW. Thousand grain weight was 34.3 g in the untreated control. Thousand grain weight with all treatments was % higher, g. With Orius+Mirador, Delaro and Proline increase in TGW was %, when it was % with Comet Pro+Prosaro, Elatus Plus+Amistar Xtra, Comet Pro+Juventus, Ascra Xpro and Elatus Era. The reason for higher yield, HLW and TGW was that with fungicide treatments the crop stayed green three to four weeks longer than in the untreated control. Contacts Study Director: Kalle Ohralahti Title: Research Scientist Organization: Address: Laboratorium, Uutetie 2 Phone No.: City+State/Prov: Jokioinen, Finland Postal Code: FI kalle.ohralahti@luke.fi Investigator: Kalle Ohralahti Title: Research Scientist Organization: Address: Laboratorium, Uutetie 2 Phone No.: City+State/Prov: Jokioinen, Finland Postal Code: FI kalle.ohralahti@luke.fi

9 18/01/18 (F ) ARM Site Description Page 3 of 4 Crop Description Crop 1: HORVS Hordeum vulgare (spring) Spring barley Variety: Voitto Depth, Unit: 5 cm Row Spacing, Unit: 12.5 cm Pest Description Pest 1 Type: D Code: PYRNTE Pyrenophora teres Common Name: Net blotch of barley Planting Date: 29/05/17 Harvest Date: 27/09/17 Harvested Width, Unit: 1.5 m Harvested Length, Unit: 8 m Harvest Equipment: Plot harvester % Standard Moisture: 15 Site and Design Treated Plot Width: 2 m Site Type: FIELD field Treated Plot Length: 8 m Experimental Unit: 1 PLOT plot Treated Plot Area: 16 m2 Treatments: 9 Tillage Type: CONTIL conventional-till Replications: 4 Study Design: RACOBL Randomized Complete Block (RC No. Previous Crop Year 1. Oat 2016 Maintenance Maintenance Rate No. Date Product Name Rate Unit 1. 29/05/17 BeFert (NPK ) 222 kg/ha 2. 29/06/17 K-Trio 1.5 L/ha % OM: 12.7 Texture: SIC silty clay ph: 5.7 Analyzed By: Agroanalyysit Element Quantity Unit P 5.5 mg/l K 215 mg/l Mg 560 mg/l Ca 3350 mg/l Soil Description Additional Measured Elements Moisture and Weather Conditions Closest Weather Station: Ilmala Distance, Unit: 10.2 km

10 18/01/18 (F ) ARM Site Description Page 4 of 4 Application Description A Application Date: 10/07/17 Appl. Start Time: 14:50 Appl. Stop Time: 15:30 Application Method: SPRAY Application Placement: FOLIAR Applied By: VR, AA, JA Air Temperature, Unit: 21.7 C % Relative Humidity: 63 Wind Velocity, Unit: 2.7 MPS Wind Direction: NE Dew Presence (Y/N): N no Soil Temperature, Unit: 18.1 C Soil Moisture: DRY % Cloud Cover: 50 Crop Stage At Each Application A Crop 1 Code, BBCH Scale: HORVS BCER Stage Scale Used: BBCH Stage Majority, Percent: 39 Stage Minimum, Percent: 37 Stage Maximum, Percent: 39 Height, Unit: 60 cm Pest Stage At Each Application A Pest 1 Code, Type, Scale: PYRNTE D Coverage, Unit: 3 % A Equipment Type: SPRAYE Operation Pressure, Unit: 2 BAR Nozzle Type: 4110 Nozzle Size: 12 Nozzle Spacing, Unit: 50 cm Nozzles/Row: 4 Boom ID: KSU2 Boom Length, Unit: 2 m Boom Height, Unit: 50 cm Carrier: WATER Spray Volume, Unit: 200 L/ha Mix Size, Unit: 2.5 liters Propellant: COMAIR Application Equipment

11 18/01/18 (F ) ARM AOV Means Table Efficacy evaluation of fungicides against net blotch in spring barley Trial ID: F Location: Jokioinen Trial Year: 2017 Protocol ID: F Investigator: Kalle Ohralahti Project ID: Advisory Trials Study Director: Kalle Ohralahti Sponsor Contact: Luke Crop Scientific Name Hordeum vulgare (spring) Hordeum vulgare (spring) Hordeum vulgare (spring) Crop Name Spring barley Spring barley Spring barley Crop Variety Voitto Voitto Voitto Pest Type D Disease D Disease Pest Code PYRNTE PYRNTE Pest Scientific Name Pyrenophora teres Pyrenophora teres Pest Name Net blotch of barley Net blotch of barley Part Rated LEAF - LEAF - LEAF - Rating Date 25/07/17 25/07/17 08/08/17 Description Rating Type PHYGEN DAMDIS DAMDIS Rating Unit % % % Sample Size, Unit 1 PLOT 1 PLOT 1 PLOT Number of Subsamples Crop Stage Majority Crop Stage Scale BBCH BBCH BBCH Trt-Eval Interval 15 DA-A 15 DA-A 29 DA-A ARM Action Codes APC APC Trt Treatment Rate Appl No. Name Rate Unit Code Untreated Check 0.0 a a 86.3 a (0%) (0%) 2 Proline 0.6 l/ha A 0.0 a 1.75 b 14.3 b (84%) (83%) 3 Comet Pro 0.4 l/ha A 0.0 a 1.50 b 2.8 c Juventus 0.4 l/ha A (86%) (97%) 4 Orius 0.8 l/ha A 0.0 a 2.38 b 11.3 b Mirador 0.4 l/ha A (78%) (87%) 5 ElatusEra 1 l/ha A 0.0 a 1.25 b 1.5 c (89%) (98%) 6 ElatusPlus 0.5 l/ha A 0.0 a 1.63 b 2.5 c Amistar Xtra 0.5 l/ha A (85%) (97%) 7 Comet Pro 0.4 l/ha A 0.0 a 1.50 b 2.3 c Prosaro 0.4 l/ha A (86%) (97%) 8 Ascra Xpro 1 l/ha A 0.0 a 1.00 b 1.3 c (91%) (99%) 9 Delaro 0.7 l/ha A 0.0 a 2.25 b 3.8 c (80%) (96%) LSD P= Standard Deviation CV Levene's F Levene's Prob(F) 0.00* 0.001* Replicate F Replicate Prob(F) Treatment F Treatment Prob(F) Means followed by same letter or symbol do not significantly differ (P=.05, Student-Newman-Keuls) Column 9: TY1 = *[5]*(100-@AVGSUB([6]))/85 Column 10: T2 = ((100-@AVGSUB([6]))*@AVGSUB([7]))/85 Column 11: T3 = ((100-@AVGSUB([6]))*@AVGSUB([8]))/85 Could not calculate LSD (% mean diff) for columns 1 because error mean square = 0.

12 18/01/18 (F ) ARM AOV Means Table Efficacy evaluation of fungicides against net blotch in spring barley Trial ID: F Location: Jokioinen Trial Year: 2017 Protocol ID: F Investigator: Kalle Ohralahti Project ID: Advisory Trials Study Director: Kalle Ohralahti Sponsor Contact: Luke Crop Scientific Name Crop Name Crop Variety Pest Type Pest Code Pest Scientific Name Pest Name Part Rated Rating Date Description Rating Type Rating Unit Sample Size, Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Trt-Eval Interval ARM Action Codes Trt Treatment No. Name 1 Untreated Check Rate Appl Rate Unit Code 2 Proline 0.6 l/ha A 3 Comet Pro 0.4 l/ha A Juventus 0.4 l/ha A 4 Orius 0.8 l/ha A Mirador 0.4 l/ha A 5 ElatusEra 1 l/ha A 6 ElatusPlus 0.5 l/ha A Amistar Xtra 0.5 l/ha A 7 Comet Pro 0.4 l/ha A Prosaro 0.4 l/ha A 8 Ascra Xpro 1 l/ha A 9 Delaro 0.7 l/ha A LSD P=.05 Standard Deviation CV Levene's F Levene's Prob(F) Replicate F Replicate Prob(F) Treatment F Treatment Prob(F) Hordeum vulgare (spring) Hordeum vulgare (spring) Hordeum vulgare (spring) Spring barley Spring barley Spring barley Voitto Voitto Voitto LEAF - GRAIN - GRAIN - 08/08/17 07/11/17 07/11/17 GREEN YIELD MOICON % kg % 1 PLOT 1 PLOT 1 PLOT BBCH BBCH BBCH 29 DA-A 29 DA-A 29 DA-A APOC APOC APOC c 4.38 b 10.6 b (100%) (100%) (100%) 82.5 b 6.05 a 10.7 a (1100%) (138%) (101%) 92.8 a 6.20 a 10.8 a (1237%) (142%) (102%) 85.0 b 5.55 a 10.8 a (1133%) (127%) (102%) 96.3 a 6.45 a 10.8 a (1283%) (147%) (102%) 96.0 a 6.13 a 10.8 a (1280%) (140%) (102%) 94.3 a 5.77 a 10.7 a (1257%) (132%) (101%) 95.8 a 6.33 a 10.8 a (1277%) (145%) (102%) 93.8 a 6.11 a 10.8 a (1250%) (139%) (102%)

13 18/01/18 (F ) ARM AOV Means Table Efficacy evaluation of fungicides against net blotch in spring barley Trial ID: F Location: Jokioinen Trial Year: 2017 Protocol ID: F Investigator: Kalle Ohralahti Project ID: Advisory Trials Study Director: Kalle Ohralahti Sponsor Contact: Luke Crop Scientific Name Crop Name Crop Variety Pest Type Pest Code Pest Scientific Name Pest Name Part Rated Rating Date Description Rating Type Rating Unit Sample Size, Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Trt-Eval Interval ARM Action Codes Trt Treatment No. Name 1 Untreated Check Rate Appl Rate Unit Code 2 Proline 0.6 l/ha A 3 Comet Pro 0.4 l/ha A Juventus 0.4 l/ha A 4 Orius 0.8 l/ha A Mirador 0.4 l/ha A 5 ElatusEra 1 l/ha A 6 ElatusPlus 0.5 l/ha A Amistar Xtra 0.5 l/ha A 7 Comet Pro 0.4 l/ha A Prosaro 0.4 l/ha A 8 Ascra Xpro 1 l/ha A 9 Delaro 0.7 l/ha A LSD P=.05 Standard Deviation CV Levene's F Levene's Prob(F) Replicate F Replicate Prob(F) Treatment F Treatment Prob(F) Hordeum vulgare (spring) Hordeum vulgare (spring) Hordeum vulgare (spring) Spring barley Spring barley Spring barley Voitto Voitto Voitto GRAIN - GRAIN - GRAIN - 07/11/17 07/11/17 07/11/17 15 % moisture 15 % moisture YIELD HLW HLW KG kg kg 1 ha 1 PLOT 1 PLOT BBCH BBCH BBCH 29 DA-A 29 DA-A 29 DA-A TY1 APOC APOC T2 APOC b 61.6 c 64.8 c (100%) (100%) (100%) 5291 a 65.6 b 68.9 b (138%) (106%) (106%) 5425 a 66.6 ab 69.9 ab (141%) (108%) (108%) 4854 a 65.6 b 68.9 b (126%) (106%) (106%) 5637 a 67.1 a 70.4 a (147%) (109%) (109%) 5360 a 66.5 ab 69.8 ab (140%) (108%) (108%) 5046 a 66.5 ab 69.8 ab (131%) (108%) (108%) 5540 a 66.9 a 70.2 a (144%) (108%) (108%) 5342 a 66.5 ab 69.8 ab (139%) (108%) (108%)

14 18/01/18 (F ) ARM AOV Means Table Efficacy evaluation of fungicides against net blotch in spring barley Trial ID: F Location: Jokioinen Trial Year: 2017 Protocol ID: F Investigator: Kalle Ohralahti Project ID: Advisory Trials Study Director: Kalle Ohralahti Sponsor Contact: Luke Crop Scientific Name Crop Name Crop Variety Pest Type Pest Code Pest Scientific Name Pest Name Part Rated Rating Date Description Rating Type Rating Unit Sample Size, Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Trt-Eval Interval ARM Action Codes Trt Treatment No. Name 1 Untreated Check Rate Appl Rate Unit Code 2 Proline 0.6 l/ha A 3 Comet Pro 0.4 l/ha A Juventus 0.4 l/ha A 4 Orius 0.8 l/ha A Mirador 0.4 l/ha A 5 ElatusEra 1 l/ha A 6 ElatusPlus 0.5 l/ha A Amistar Xtra 0.5 l/ha A 7 Comet Pro 0.4 l/ha A Prosaro 0.4 l/ha A 8 Ascra Xpro 1 l/ha A 9 Delaro 0.7 l/ha A LSD P=.05 Standard Deviation CV Levene's F Levene's Prob(F) Replicate F Replicate Prob(F) Treatment F Treatment Prob(F) Hordeum vulgare (spring) Hordeum vulgare (spring) Spring barley Voitto Spring barley Voitto GRAIN - GRAIN - 15/11/17 15/11/17 15 % moisture TKW TKW g g 1 PLOT 1 PLOT BBCH BBCH 128 DA-A 128 DA-A APOC T3 APOC d d (100%) (100%) abc abc (119%) (119%) abc abc (123%) (123%) c c (117%) (117%) a a (126%) (126%) abc abc (123%) (123%) abc abc (122%) (122%) ab ab (125%) (125%) bc bc (118%) (118%)