LAMPIRAN 8
9 Lampiran 1 Komposisi media pertumbuhan yang digunakan A. Media nutrien agar (NA) - Agar 1.5% - Nutrient broth 0.8% B. Media King s B agar - Agar 1.5% - Pepton 2% - K 2 HPO 4 0.15% - MgSO 4.4H 2 O 0.15% - Gliserol 1.5% C. Media luria agar (LA) - Agar 1.5% - NaCl 1% - Tripton 1% - Yeast extract 0.5%
10 Lampiran 2 Hasil sekuensing dan analisis bioinformatika gen aiia dan 16S rrna dengan program BLAST-N dan BLAST X A. Urutan nukleotida gen aiia isolat INT1c 1 AAAAACACACAAACCTTACGTACTATCAGTTAAACGAAGATAGTTCAAAAAAAGTTTAT 70 71 AATTTATTTAAAAAAATTATTTAAACGAAAAAGTCATGAGCACGCGAACTCATGACTTTT 130 131 TGCACTATATATACTCAGGGAACACTCTACAACCCTTTTCCTGCTCTATATCATGACCAA 190 191 AGAAAACAATTGGTTTCTCTTTCGCCACAACTCCTTTTAAACGTTTAATTGAAGATAAAG 250 251 CTAATTCTGGATCAAATCCTGCGTTAAACGGCACTTCATCTTCAAAATTCTCTTTCGTGT 310 311 ACGATGCATCAATTGTTAATAAAACTGAGCCGGATTGCTCCGTCTCCAAATGAATAGCGA 370 371 CTGATAGGCCTGGAGAATGAATTCCCTGGCGCGAGTATACAATTAATTGAACACCTGGTA 430 431 CCACTTCATAATCCCCTTCAATAATTTTGTAGTTCAAATGCGGTAATATACATTCTTTCA 490 491 TATATTCTTCTCTATG 495 B. Analisis sekuen gen aiia isolat INT1c dengan program BLAST-N > gb AE016877.1 Bacillus cereus ATCC 14579, complete genome Length=5411809 Features in this part of subject sequence: hypothetical protein N-acyl homoserine lactone hydrolase Score = 798 bits (432), Expect = 0.0 Identities = 470/485 (97%), Gaps = 15/485 (3%) Strand=Plus/Plus Query 11 AAACCTTACGTACTATCAGTTAAACGAAGATAGTTCAAAAAAAGTTTATAATTTATTTAA 70 Sbjct 3409374 AAACCTTACGTACTATCAGTTAAACGAAGATAGTTCAAAAAAAGTTTATAATTTATTTAA 3409433 Query 71 AAAAATTATTTAAACGAAAAAGTCATGAGCACGCGAACTCATGACTTTTTGCACTATATA 130 Sbjct 3409434 AAAAATTATTTAAACGAAAAAGTCATGAGCACGCGAACTCATGACTTTTTGCACTATATA 3409493 Query 131 TACTCAGGGAACACTCTACAACCCTTTTCCTGCTCTATATCATGACCAAAGAAAACAATT 190 Sbjct 3409494 TACTCAGGGAACACTCTACAACCCTTTTCCTGCTCTATATCATGACCAAAGAAAACAATT 3409553 Query 191 GGTTTCTCTTTCGCCACAACTCCTTTTAAACGTTTAATTGAAGATAAAGCTAATTCTGGA 250 Sbjct 3409554 GGTTTCTCTTTCGCCACAACTCCTTTTAAACGTTTAATTGAAGATAAAGCTAATTCTGGA 3409613 Query 251 TCAAATCCTGCGTTAAACGGCACTTCATCTTCAAAATTCTCTTTCGTGTACGATGCATCA 310 Sbjct 3409614 TCAAATCCTGCG--AA-CGGCACTTCATCTTCAAAATTCTCTTTCGTGTACGATGCATCA 3409670 Query 311 ATTGTTAATAAAACTGAGCCGGATTGCTCCGTCTCCAAATGAATAGCGACTGATAGGCCT 370 Sbjct 3409671 ATTGTTAATAAAACTGAGCCGGATTGCTCCGTCTC-AA-TGAATAGCGACTGAT-GGCCT 3409727 Query 371 GGAGAATGAATTCCCTGGCGCGAGTATACAATTAATTGAACACCTGGTACCACTTCATAA 430 Sbjct 3409728 GGAGAATGA---CC-TGGCG----TATACAAT-AATTGAACACCTGGTACCACTTCATAA 3409778 Query 431 TCCCCTTCAATAATTTTGTAGTTCAAATGCGGTAATATACATTCTTTCATATATTCTTCT 490 Sbjct 3409779 TCCCCTTCAATAATTTTGTAGTTCAAATGCGGTAATATACATTCTTTCATATATTCTTCT 3409838 Query 491 CTATG 495 Sbjct 3409839 CTATG 3409843
11 C. Urutan nukleotida gen aiia isolat SGT3g 1 CGGGATCCCATGACCAGTAAAAGAAGGCTTTTATTTTCCCCCCCCCCCC 49 50 TCCCAGCAAGGTCCGTTGTATTGGTTAGATCAATTCTTTCTGTTAATTAGTACACTCCGC 109 110 GCCGGGGGAATTTTATTGAACTTTACCTGTATGGTGTTATCTTTTTGGAGACAGAAGAGG 169 170 GGCCTATTTTAGTAGATACAGGTATGCCAGAAAGTGCAGTTAATAATGAAGGGATTTTTA 229 230 ACGGTACATTTGTTGAAGGACAGATTTTACCGAAAATGACTGAAGAAGATAGAATCGTGA 289 290 ATATATTAAAGCGTGTAGGGTATGAGCCGGACGACC-TTT-TATATTATTAGTTCTCACT 347 348 TACATTTTGATCATGCAGGAGGAAACGGTGCTTTTACAAATACACCGATTATTGTGCAGC 407 408 GAGCGGAATATGAGGCAGCACTTCATAGAGAAGAATATATGAAAGAATGTATATTACCGC 467 468 ATTTGAACTACAAAATTATTGAAGGGGATTATGAAGTGGTACCAGGTGTTCAATTATTGT 527 528 ATACGCCAGGTCATTCTCCAGGCCATCAGTCGTTATTCATTGAGACGGAGCAATCCGGTT 587 588 CAGTTTTATTAACAATTGATGCATCGTACACGAAAGAGAATTTTGAAGATGAAGTGCCGT 647 648 TCGCAGGATTTGATCCAGAATTAGCTTTATCTTCAATCAAACGCTTAAAAGAAGTTGTGA 707 708 CAAAAGAGAAATCGATTGTTTTCTTTGGTCATGATATAGAGCAGGAAAAAGGGTTGTAGA 767 768 GTGTTCCCGGAGTATATATAGTGCAAAAAGTCATGAGTTCGCGTGCTCATGACTTTTTCG 827 828 TTTAAATAATTTTTTTTAAATAAATTATAAACTTTTTTTCAAACTATCTTCCGTTTAACT 887 888 GGATAGTACGTAAAGTTTTTACATCAATTAAGGAGTATCCTTG 930 D. Analisis sekuen gen aiia isolat SGT3g dengan program BLAST-N > gb CP001903.1 Bacillus thuringiensis BMB171, complete genome Length=5330088 Features in this part of subject sequence: hypothetical protein N-acyl homoserine lactone hydrolase Score = 1404 bits (760), Expect = 0.0 Identities = 846/883 (96%), Gaps = 24/883 (3%) Strand=Plus/Minus Query 50 TCCCAGCAAGGTCCGTTGTATTGGTTAGATCAATTCTTTCTGTTAATTAGTACACTCCGC 109 Sbjct 3334673 TCCCAGC-AGGT-CGTTGTA-T-GTTAGATC-ATTC-TTCTGTTAA-TGGTACACT-CGC 3334622 Query 110 GCCGGGGGAATTTTATTGAACTTTACCTGTATGGTGTTATCTTTTTGGAGACAGAAGAGG 169 Sbjct 3334621 GCC-GGGGAA-TTTATTGAAC-TTACCTGTATGGTGTTATC-TTTTGGAGACAGAAGAGG 3334566 Query 170 GGCCTATTTTAGTAGATACAGGTATGCCAGAAAGTGCAGTTAATAATGAAGGGATTTTTA 229 Sbjct 3334565 GGCCTATTTTAGTAGATACAGGTATGCCAGAAAGTGCAGTTAATAATGAAGGGCTTTTTA 3334506 Query 230 ACGGTACATTTGTTGAAGGACAGATTTTACCGAAAATGACTGAAGAAGATAGAATCGTGA 289 Sbjct 3334505 ACGGTACATTTGTTGAAGGACAGATTTTACCGAAAATGACTGAAGAAGATAGAATCGTGA 3334446 Query 290 ATATATTAAAGCGTGTAGGGTATGAGCCGGACGACC-TTT-TATATTATTAGTTCTCACT 347 Sbjct 3334445 ATATATTAAAGCGTGTAGGGTATGAGCCGGACGACCTTTTATATATTATTAGTTCTCACT 3334386 Query 348 TACATTTTGATCATGCAGGAGGAAACGGTGCTTTTACAAATACACCGATTATTGTGCAGC 407 Sbjct 3334385 TACATTTTGATCATGCAGGAGGAAACGGTGCTTTTACAAATACACCGATTATTGTGCAGC 3334326 Query 408 GAGCGGAATATGAGGCAGCACTTCATAGAGAAGAATATATGAAAGAATGTATATTACCGC 467 Sbjct 3334325 GAACGGAATATGAGGCAGCACTTCATAGAGAAGAATATATGAAAGAATGTATATTACCGC 3334266 Query 468 ATTTGAACTACAAAATTATTGAAGGGGATTATGAAGTGGTACCAGGTGTTCAATTATTGT 527 Sbjct 3334265 ATTTGAACTACAAAATTATTGAAGGGGATTATGAAGTGGTACCAGGTGTTCAATTATTGT 3334206
12 Query 528 ATACGCCAGGTCATTCTCCAGGCCATCAGTCGTTATTCATTGAGACGGAGCAATCCGGTT 587 Sbjct 3334205 ATACGCCAGGTCATTCTCCAGGCCATCAGTCGTTATTCATTGAGACGGAGCAATCCGGTT 3334146 Query 588 CAGTTTTATTAACAATTGATGCATCGTACACGAAAGAGAATTTTGAAGATGAAGTGCCGT 647 Sbjct 3334145 CAGTTTTATTAACAATTGATGCATCGTACACGAAAGAGAATTTTGAAGATGAAGTGCCGT 3334086 Query 648 TCGCAGGATTTGATCCAGAATTAGCTTTATCTTCAATCAAACGCTTAAAAGAAGTTGTGA 707 Sbjct 3334085 TCGCAGGATTTGATCCAGAATTAGCTTTATCTTCAATTAAACGTTTAAAAGGAGTTGTGG 3334026 Query 708 CAAAAGAGAAATCGATTGTTTTCTTTGGTCATGATATAGAGCAGGAAAAAGGGTTGTAGA 767 Sbjct 3334025 CGAAAGAGAAACCAATTGTTTTCTTTGGTCATGATATAGAGCAGGAAAA-GGGTTGTAGA 3333967 Query 768 GTGTTCCCGGAGTATATATAGTGCAAAAAGTCATGAGTTCGCGTGCTCATGACTTTTTCG 827 Sbjct 3333966 GTGTTCCCTGAGTATATATAGTGCAAAAAGTCATGAGTTCGCGTGCTCATGACTTTTTCG 3333907 Query 828 TTTAAATAATTTTTTTTAAATAAATTATAAACTTTTTTTCAAACTATCTTCCGTTTAACT 887 Sbjct 3333906 TTTAAATAATTTTTTT-AAATAAATTATAAACTTTTTTTGAA-CTATCTTC-GTTTAACT 3333850 Query 888 GGATAGTACGTAAAGTTTTTACATCAATTAAGGAGTATCCTTG 930 Sbjct 3333849 G-ATAGTACGTAA-GTTTT-ACATCA-TCA-GGAGTATC-TTG 3333813 E. Analisis sekuen gen aiia isolat INT1c dengan program BLAST-X > pdb 2BR6 A Chain A, Crystal Structure Of Quorum- Quenching N-Acyl Homoserine Lactone Lactonase Score = 197 bits (500), Expect = 3e-51 Identities = 102/123 (83%), Positives = 108/123 (88%), Gaps = 5/123 (4%) Frame = -1 Query 495 HREEYMKECILPHLNYKIIEGDYEVVPGVQLIVYSRQGIHSPGLSVAIHLETEQSGSVLL 316 HREEYMKECILPHLNYKIIEGDYEVVPGVQL+ HSPG ++ ++TEQSGSVLL Sbjct 135 HREEYMKECILPHLNYKIIEGDYEVVPGVQLLYTPG---HSPGHQ- SLFIKTEQSGSVLL 190 Query 315 TIDASYTKENFEDEVPFNAGFDPELALSSIKRLKGVVAKEKPIVFFGHDIEQEKGCRVFP 136 TIDASYTKENFEDEVPF AGFDPELALSSIKRLK VV KEKPI+FFGHDIEQEK CRVFP Sbjct 191 TIDASYTKENFEDEVPF- AGFDPELALSSIKRLKEVVKKEKPIIFFGHDIEQEKSCRVFP 249 Query 135 EYI 127 EYI Sbjct 250 EYI 252
13 F. Analisis sekuen gen aiia isolat INT1c dengan program BLAST-X > pdb 2BR6 A Chain A, Crystal Structure Of Quorum- Quenching N-Acyl Homoserine Lactone Lactonase pdb 2BTN A Chain A, Crystal Structure And Catalytic Mechanism Of The Quorum- Quenching N-Acyl Homoserine Lactone Hydrolase Length=252 Sort alignments for this subject sequence by: E value Score Percent identity Query start position Subject start position Score = 285 bits (728), Expect(4) = 1e-109 Identities = 138/143 (97%), Positives = 140/143 (98%), Gaps = 0/143 (0%) Frame = +2 Query 329 YIISSHLHFDHAGGNGAFTNTPIIVQRAEYEAALHREEYMKECILPHLNYKIIEGDYEVV 508 YIISSHLHFDHAGGNGAFTNTPIIVQR EYEAALHREEYMKECILPHLNYKIIEGDYEVV Sbjct 101 YIISSHLHFDHAGGNGAFTNTPIIVQRTEYEAALHREEYMKECILPHLNYKIIEGDYEVV 160 Query 509 PGVQLLYTPGHSPGHQSLFIETEQSGSVLLTIDASYTKENFEDEVPFAGFDPELALSSIK 688 PGVQLLYTPGHSPGHQSLFI+TEQSGSVLLTIDASYTKENFEDEVPFAGFDPELALSSIK Sbjct 161 PGVQLLYTPGHSPGHQSLFIKTEQSGSVLLTIDASYTKENFEDEVPFAGFDPELALSSIK 220 Query 689 RLKEVVTKEKSIVFFGHDIEQEK 757 RLKEVV KEK I+FFGHDIEQEK Sbjct 221 RLKEVVKKEKPIIFFGHDIEQEK 243 Score = 123 bits (308), Expect(4) = 1e-109 Identities = 59/60 (98%), Positives = 60/60 (100%), Gaps = 0/60 (0%) Frame = +1 Query 154 LETEEGPILVDTGMPESAVNNEGIFNGTFVEGQILPKMTEEDRIVNILKRVGYEPDDLLY 333 LETEEGPILVDTGMPESAVNNEG+FNGTFVEGQILPKMTEEDRIVNILKRVGYEPDDLLY Sbjct 42 LETEEGPILVDTGMPESAVNNEGLFNGTFVEGQILPKMTEEDRIVNILKRVGYEPDDLLY 101 Score = 25.4 bits (54), Expect(4) = 1e-109 Identities = 9/12 (75%), Positives = 11/12 (92%), Gaps = 0/12 (0%) Frame = +3
14 Query 750 RKKGCRVFPEYI 785 ++K CRVFPEYI Sbjct 241 QEKSCRVFPEYI 252 Score = 24.6 bits (52), Expect(4) = 1e-109 Identities = 14/33 (42%), Positives = 16/33 (48%), Gaps = 7/33 (21%) Frame = +3 Query 90 C*LVHSA------PGEFY*TLPVWCYLFGDRRG 170 C L HS+ PG+ LPVWCYL G Sbjct 16 CMLDHSSVNSALTPGKLL-NLPVWCYLLETEEG 47 G. Urutan nukleotida gen 16S rrna isolat INT1c 1 GGAGAATGATAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGT 61 62 AACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAACATTTT 121 122 GAACCGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGT 181 182 CGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAG 241 242 AGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCCTACGGGGAAGGCAGCC 301 302 AGTAGGGAATCTTCCGCAATGGAACGATAAGTTCTGACGGAGCAACCGCCGGCGTGAGTG 361 362 ATGAAGGCCTTTCGGGTTCGTAAAACTCTGTTGTTTAGGGAAAGAACAAGTGCTTAGTTG 421 422 AATAAGCTGGCACCTTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCA 481 482 GCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA 541 542 GGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAAC 601 602 TGGGGAGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTA 661 662 GAGATATGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGC 721 722 GCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGA 781 782 GTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCG 841 813 GTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCG 872 842 CCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCG 901 902 GTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTC 961 962 TGAAAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAGTGACAGGTGGTGCATGGTTG 1021 1022 TCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCT 1081 1082 TAGTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGG 1141 1142 TGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGG 1201 1202 ACGGTACAAAGAGCTGCAAGACCGCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTT 1261 1262 CGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCA 1322 H. Analisis sekuen gen 16S rrna isolat INT1c dengan program BLAST-N > gb JF837192.1 Bacillus cereus strain GM3 16S ribosomal RNA gene, partial sequence Length=1363 Score = 2324 bits (1258), Expect = 0.0 Identities = 1303/1321 (99%), Gaps = 18/1321 (1%) Strand=Plus/Plus Query 4 GAAT-GA-TAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGT 61 Sbjct 49 GAATGGATTAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGT 108 Query 62 AACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAACATTTT 121 Sbjct 109 AACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAACATTTT 168 Query 122 GAACCGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGT 181 Sbjct 169 GAACCGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGT 228 Query 182 CGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAG 241 Sbjct 229 CGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAG 288 Query 242 AGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCCTACGGGGAAGGCAGCC 301
15 Sbjct 289 AGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACT-CCTAC-GGG-AGGCAG-C 344 Query 302 AGTAGGGAATCTTCCGCAATGGAACGATAAGTTCTGACGGAGCAACCGCCGGCGTGAGTG 361 Sbjct 345 AGTAGGGAATCTTCCGCAATGG-ACGA-AAG-TCTGACGGAGCAA-CGCC-GCGTGAGTG 399 Query 362 ATGAAGGCCTTTCGGGTTCGTAAAACTCTGTTGTTTAGGGAAAGAACAAGTGCTTAGTTG 421 Sbjct 400 ATGAAGG-CTTTCGGG-TCGTAAAACTCTGTTG-TTAGGG-AAGAACAAGTGC-TAGTTG 454 Query 422 AATAAGCTGGCACCTTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCA 481 Sbjct 455 AATAAGCTGGCACC-TTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCA 513 Query 482 GCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA 541 Sbjct 514 GCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA 573 Query 542 GGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAAC 601 Sbjct 574 GGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAAC 633 Query 602 TGGGGAGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTA 661 Sbjct 634 T-GGGAGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTA 692 Query 662 GAGATATGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGC 721 Sbjct 693 GAGATATGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGC 752 Query 722 GCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGA 781 Sbjct 753 GCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGA 812 Query 782 GTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCG 841 Sbjct 813 GTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCG 872 Query 842 CCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCG 901 Sbjct 873 CCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCG 932 Query 902 GTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTC 961 Sbjct 933 GTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTC 992 Query 962 TGAAAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAGTGACAGGTGGTGCATGGTTG 1021 Sbjct 993 TGAAAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAGTGACAGGTGGTGCATGGTTG 1052 Query 1022 TCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCT 1081 Sbjct 1053 TCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCT 1112 Query 1082 TAGTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGG 1141 Sbjct 1113 TAGTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGG 1172 Query 1142 TGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGG 1201 Sbjct 1173 TGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGG 1232 Query 1202 ACGGTACAAAGAGCTGCAAGACCGCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTT 1261 Sbjct 1233 ACGGTACAAAGAGCTGCAAGACCGCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTT 1292 Query 1262 CGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGC 1321 Sbjct 1293 CGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGC 1352 Query 1322 A 1322 Sbjct 1353 A 1353
16 I. Urutan nukleotida gen 16S rrna isolat SGT3g 1 GGGGGGGAAGGTTAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGGTGAGTAACACG 71 72 TGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAAC 131 132 ATTTTGAACCGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACC 191 192 CGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGAC 251 252 CTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAG 311 312 CAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGA 371 372 AGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTG 431 432 GCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAA 491 492 TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCT 551 552 GAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGA 611 612 GGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGG 671 672 TAAGTCTGATGTCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTG 731 732 GGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGT 791 792 TAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTA 851 852 CGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGT 911 912 GGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTA 971 972 GAGATAGGGCTTCTCCTTCGGGAGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCG 1031 1032 TGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCATC 1091 1040 ATTAAGTTGGGCTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCATC 1099 1092 ATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAAGAAGGTGGGGATGACG 1151 1152 TCAAATCATCATGCCCCTTAAGACCTGGGCTACCCACGTGCTACAATGGACGGTTCAAAA 1211 1212 AGCTGCAAGACCGCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTTCGGATTGTAGG 1269 1270 CTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGNTCAGCAGCCGC 1313 J. Analisis sekuen gen 16S rrna isolat SGT3g dengan program BLAST-N > gb JF496321.1 Bacillus thuringiensis strain A1-1 16S ribosomal RNA gene, partial sequence Length=1411 Score = 2364 bits (1280), Expect = 0.0 Identities = 1296/1304 (99%), Gaps = 2/1304 (0%) Strand=Plus/Plus Query 12 TTAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGC 71 Sbjct 20 TTAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGC 79 Query 72 CCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAACATTTTGAACCGC 131 Sbjct 80 CCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAACATTTTGAACCGC 139 Query 132 ATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTA 191 Sbjct 140 ATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTA 199 Query 192 GCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGA 251 Sbjct 200 GCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGA 259 Query 252 TCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATC 311 Sbjct 260 TCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATC 319 Query 312 TTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGT 371 Sbjct 320 TTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGT 379 Query 372 CGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGG 431 Sbjct 380 CGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGG 439 Query 432 TACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGC 491 Sbjct 440 TACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGC 499 Query 492 AAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGT 551 Sbjct 500 AAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGT 559
17 Query 552 GAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGA 611 Sbjct 560 GAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGA 619 Query 612 GGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGG 671 Sbjct 620 GGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGG 679 Query 672 CGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAG 731 Sbjct 680 CGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAG 739 Query 732 GATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCC 791 Sbjct 740 GATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCC 799 Query 792 GCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGC 851 Sbjct 800 GCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGC 859 Query 852 TGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA 911 Sbjct 860 TGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA 919 Query 912 AGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTAGAGATAGGGCTT 971 Sbjct 920 AGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTAGAGATAGGGCTT 979 Query 972 CTCCTTCGGGAGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATG 1031 Sbjct 980 CTCCTTCGGGAGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATG 1039 Query 1032 TTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCATCATTAAGTTGGGC 1091 Sbjct 1040 TTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCATCATTAAGTTGGGC 1099 Query 1092 ACTCTAAGGTGACTGCCGGTGACAAACCGGAAGAAGGTGGGGATGACGTCAAATCATCAT 1151 Sbjct 1100 ACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCAT 1159 Query 1152 GCCCCTTAAGACCTGGGCTACCCACGTGCTACAATGGACGGTTCAAAAAGCTGCAAGACC 1211 Sbjct 1160 GCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAGCTGCAAGACC 1219 Query 1212 GCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCC 1271 Sbjct 1220 GCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCC 1279 Query 1272 TACATGAAGCTGGAATCGCTAGTAATCGCGN-TCAGCA-GCCGC 1313 Sbjct 1280 TACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGC 1323