Supplementary Material : Baker et al. 1

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1 Supplementary Material : Baker et al. 1 Supplementary Material Title: Species identity and human consumption of beaked whales in the Gilbert Islands, Republic of Kiribati Authors: C. Scott Baker, Al Hutt, Kirsten Thompson, Merel L. Dalebout, Judith Robins, Robert L. Brownell Jr. and Greg S. Stone SupTable 1: Sequences of the mtdna control region (DLP) used in species identification of bones from Gilbert Islands. Shown in FASTA format with sample code followed by primer information. >KI002 Dlp1.5 to Dlp4R CATAAACTATTCCCTGAAAAGTCTTGTTATAGAATCACTATAACCCCACAGTACTACGTCAGTATTGAAAAAAA AAATCCTACAGTACATTTACTGTATTAATTATAGAGGCACACCTACCTACACGCTAATGTATAGCGCCTCTCT AGGACTGTATGTATATATATACTATGTATAACTGTGCATTCATTTATTTTCACTACGGAGAGTTAAAGCTCGTA ATTAATTTTTTTAATTTTACATAAGTACATAATTTGCATTATTCGTACATGTGCCCGTTCCATTAAATCACAAGC >KI011 Dlp1.5 to Dlp4R CATAAACTATTCCCTGAAAAAGTCTTATTGTAGAATCACCACAACCCTACAGTGCTATGTCAGTATTGAAAAAA AATATCCTACAGTACATTTACTGTATTAATCATACAAGCATACTTACCTACACGCTAATATATAGCGTCTCTCC AAGACTGTATGTATATATATACTATGTATAACTGTGCATTCATTTATTTTCACTACGGAGAGTTAAAGCTCGTA ATTAATTTTTTTTATTTTACATAAGTACATAATTTGCATTACTTGTACATGTGCCCGTTCCATTAGATCACGAGC >KI012 Dlp1.5 to Dlp4R AACTATTCCCTGAAAAAGTCTTATTGTAGAATCACCACAACCCTACAGTGCTATGTCAGTATTGAAAAAAAATA TCCTACAGTACATTTACTGTATTAATCATACAAGCATACTTACCTACACGCTAATATATAGCGTCTCTCCAAGA CTGTATGTATATATATACTATGTATAACTGTGCATTCATTTATTTTCACTACGGAGAGTTAAAGCTCGTAATTAA TTTTTTTTATTTTACATAAGTACATAATTTGCATTACTTGTACATGTGCCCGTTCCATTAGATCACGAGCTTAAT CACCATGCCG >KI018 Dlp1.5 to Dlp4R ATTCCCTGAAAAGTCTTGTTATAGAATCACTATAACCCCACAGTACTACGTCAGTATTGAAAAAAAAAATCCTA CAGTACATTTACTGTATTAATTATAGAGGCACACCTACCTACACGCTAATGTATAGCGCCTCTCTAGGACTGT ATGTATATATATACTATGTATAACTGTGCATTCATTTATTTTCACTACGGAGAGTTAAAGCTCGTAATTAATTTT TTTAATTTTACATAAGTACATAATTTGCATTATTCGTACATGTGCCCGTTCCATTAAATCACGAGCTTAATCAC CATGCCG >KI019 Dlp1.5 to Dlp4R CATAAACTATTCCCTGAAAAGTCTTGTTATAGAATCACTATAACCCCACAGTACTACGTCAGTATTGAAAAAAA AAATCCTACAGTACATTTACTGTATTAATTATAGAGGCACACCTACCTACACGCTAATGTATAGCGCCTCTCT AGGACTGTATGTATATATATACTATGTATAACTGTGCATTCATTTATTTTCACTACGGAGAGTTAAAGCTCGTA ATTAATTTTTTTAATTTTACATAAGTACATAATTTGCATTATTCGTACATGTGCCCGTTCCATTAAATCACGAGC >UKiribati_Dlp300bp GAAAAAGTCTTATTGTAGAATCACCACAACCCTACAGTGCTATGTCAGTATTGAAAAAAAATATCCTACAGTA CATTTACTGTATTAATCATACAAGCATACTTACCTACACGCTAATATATAGCGTCTCTCCAAGACTGTATGTAT ATATATACTATGTATAACTGTGCATTCATTTATTTTCACTACGGAGAGTTAAAGCTCGTAATTAATTTTTTTTAT TTTACATAAGTACATAATTTGCATTACTTGTACATGTGCCCGTTCCATTAGATCACGAGCTTAATCACCATGCC GCGT >KI024_dlp1.5 to Dlp5R CCTTATTGTAAATAACCACAAACCTCTAGGGCTATGTCAGTATTAAAATTAATCTAGTCCAATTACATTCTCAT CGTTAAAAAACCATACAAACATACACCCCATCCAATAAAATAGCGTTCTCCTCGTAGATGTATGTATATACATA GCTATGTATAATAGTGCATTCAATTATTTTCCACACGAGAAGTTAAAGCCCGTATTAGATTTTATTAATTTTACA TATTACATAATATTATTGATCGTACATAAGACATACTTCTAAATCAGTTCCAGTCCCTTAACAGTATGGCCGCT CCATTAGATCACGAGCTTAACCACCATGCCGCGTGAAAANNNNAACCCGCNANGCTGGGATCCCCCTCCTC GCACCGGGCCCATTAACTGTGGGGGTAGCTATTTTATGCCTTTTACAATACATCTGGTTCTTACTTCAGGGC CATATTCACTTAAAATCGCCCACTCGTTCCTCTTAAATAAGACAT

2 Supplementary Material : Baker et al. 2 SupTable 2: Sequences of the mtdna cytochrome b used in species identification of bones from Gilbert Islands. Shown in FASTA format with sample code followed by primer information. >KI002_CybMLDF to Cyb R AAATATTTCCTCATGATGAAATTTCGGCTCCTTACTCGGCCTCTGCCTAATTATACAAATTCTCACAGGACTAT TCCTGGCAATACACTACACACCAGACACCACAACAGCCTTTTCATCTGTTACACATATCTGCCGAGACGTTA ACTATGGCTGAATTATCCGATATCTACATGCAAATGGGGCTTCTATATTTTTNNNNNNNNNNNATGCACATAT TGGACGTGGTCTGTACTACGGCTCTTATATCTTCCAAGAAACATGAAATATCGGAGTAATCTTACTCTTTACA GTTATAGCCACTGCATTTGTAGGCTATGTCCTACCAT > KI009_CybMLDF to Cyb R ATCTCCTCATGATGAAACTTCGGCTCCTTACTCGGCCTCTGCCTCATCATACAAATTCTCACAGGCCTGTTCT TAGCAATACACTATACACCAGACACAACAACAGCCTTCTCATCCGTTGCACACATTTGCCGAGACGTCAACT ATGGCTGAATCATTCGATACCTACACGCAAACGGGGCCTCCATATTCTTCATCTGCCTTTACGCCCATATCG GACGTGGACTATATTACGGCTCCTATATCTTTCAAGAAACATGAAACATCGGAGTAATCCTACTCCTTGCAGT TATAGCTACCGCATTTGTGGGCTATGTCCTACCTT

3 Supplementary Material : Baker et al. 3 SupFigure 1: Whaling artifacts and cetacean bones collected during surveys of the Gilbert Islands from 18 June to 14 July 2009: A) whaling try-pot, a remnant of 19th century whaling contact; B) vertebra (KI004, identified as Mesoplodon sp., an unrecognized species or subspecies) and rib bone (KI014, no DNA identification) collected on Onotoa Island; and C) mandible, rib and teeth of a sperm whale on display in Butaritari Island. Photographs courtesy of Al Hutt. A B C

4 Supplementary Material : Baker et al. 4 SupFigure 2: Representative identification of mtdna sequences from cetacean material collected from the outer islands of the Gilbert group, in the Republic of Kiribati, using reference sequences available through the web-based application, DNA-surveillance (Ziphiidae Vs4.3): A) control region (DLP) of KI0o2, KI011, KI012, KI018 and KI019; B) cytochrome b (CYB) of KI002 and KI009. The neighbor-joining tree reflects species-specific grouping with bootstrap values based on 500 replications. The program is available at A) Control region Berardius bairdii Berardius arnuxii Mesoplodon bowdoini Tasmacetus shepherdi Mesoplodon carlhubbsi Mesoplodon stejnegeri Indopacetus pacificus Mesoplodon traversii Mesoplodon densirostris Mesoplodon perrini Mesoplodon peruvianus Mesoplodon grayi Mesoplodon hectori Mesoplodon layardii Mesoplodon bidens Mesoplodon ginkgodens Mesoplodon sp. Mesoplodon europaeus Mesoplodon mirus Ziphius cavirostris Hyperoodon ampullatus Hyperoodon planifrons Kogia breviceps

5 Supplementary Material : Baker et al. 5 B) Cytochrome b Mesoplodon bowdoini Mesoplodon traversii Mesoplodon bidens Mesoplodon layardii Mesoplodon mirus Mesoplodon hectori Mesoplodon ginkgodens Mesoplodon europaeus Mesoplodon perrini Mesoplodon peruvianus Mesoplodon stejnegeri Mesoplodon grayi Mesoplodon densirostris Berardius arnuxii Berardius bairdii Mesoplodon carlhubbsi Tasmacetus shepherdi Hyperoodon ampullatus Hyperoodon planifrons Ziphius cavirostris Indopacetus pacificus Kogia breviceps