Vesien nopea mikrobivalvonta ja biotekninen puhdistaminen

Transkriptio

1 Vesien nopea mikrobivalvonta ja biotekninen puhdistaminen Tietoa huipulta seminaari Kuopio Finnoflag Oy, toimitusjohtaja Elias Hakalehto

2 PMEU-TEKNOLOGIAN TAUSTA-AJATUKSIA JOTTA VESINÄYTTEIDEN MIKROBIMÄÄRIÄ VOITAISIIN LUOTETTAVASTI TUTKIA: NÄYTTEET OTETTAVA EDUSTAVALLE TAVALLA RIKASTUSVILJELY ALOITETTAVA HETI OIKEAT OLOSUHTEET JA SELEKTIO (ESIM. HYGIENIAINDIKAATTORIT) ETÄSEURANTA VEDENJAKELUVERKOSTOSSA TAI TEOLLISUUDEN TUOTANTOLINJASTOILLA TAI EPIDEMIOIDEN YHTEYDESSÄ REAALIAIKAINEN RIKASTUSVILJELYN TULOSSEURANTA JATKONÄYTTEIDEN VERIFIKAATIO/KONFIRMAATIO PMEU ON YHTEENSOPIVA ERI GENEETTISTEN JA IMMUNOLOGISTEN VALVONTAMENETELMIEN KANSSA A. TALOUSVESI B. TEOLLISUUDEN KÄYTTÖVESI C. UIMA- JA VIRKISTYSVEDET D. LUONNONVEDET E. RISKITEKIJÖIDEN (ESIM. ANTIBIOOTTIRESISTENTIT KANNAT) MONITOROINTI

3 PMEU (Portable Microbe Enrichment Unit) versiot PMEU Spectrion Ruiskut PMEU:ssa PMEU Scentrion PMEU varustettuna automaattisella näytteenotolla

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9 PMEU teknologia POLARIS tutkimuksessa veden laadun verifioinnissa PMEU Spectrion The server of Polaris Internet Data updated every 10 minutes

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11 MILESTONES OF FINNOFLAG TECHNOLOGY 1993 Finnoflag Oy established 1995 Patent for Syringe Sampler 1997 Bioprocess research started 1998 Patent for Enrichment Principle, First Prototype of PMEU PMEU Prototypes in Medica, Dûsseldorff PMEU Developments (Finnoflag and Savonia) 2003 First Commercial PMEU Versions 2005 First Prototype of PMEU Spectrion 2006 Biorefinery Tests Based on Metabolic Studies Started in Finnoflag Oy Laboratory 2007 First Biorefinery Patents 2007 First Prototype of PMEU Scentrion 2008 Hospital Validation of PMEU Technology Initiated 2009 Samplion Oy Established for PMEU Manufacturing 2010 Laboratory Testing of Coliline PMEU Principle 2011 Polaris Project Tests at Water Departments 2012 Coliline PMEU for Water Departments Licenced to Berner Oy ABOWE Project, Savonia as Lead Partner, Finnoflag Oy as Principal Technology Provider for Pilot A Industrial Biorefinery Projects for Finnish and Overseas Customers

12 PMEU IN ENVIRONMENTAL STUDIES: SOURCE TRACKING 1/2 The use of faecal enterococci in lake water monitoring programmes is often limited to hygienic questions. Typing of bacterial strains with a biochemical typing method (PhenePlate TM ) gives possibilities for source tracking of waste waters. Enhanced sample enrichment with portable microbe enrichment unit (PMEU) extends the possibilities of bacterial studies even in situations of environmentally harsh conditions. The phenotypes of faecal enterococci were studied in urban and forest industry waste waters and the results were compared to phenotypes found in Lake Kallavesi, Central Finland, which is the recipient water body.

13 PMEU IN ENVIRONMENTAL STUDIES: SOURCE TRACKING 2/2 Proportion of enterococci cgf-group (blue) and E. faecalis/e. faecium (green) in Lake Kallavesi near forest industry and urban sewage treatment plants. Heitto, L., Heitto, A., & Hakalehto, E. (2009). Tracing wastewaters with faecal enterococci. (Poster). Second European Large Lakes Symposium. Norrtälje, Sweden.

14 PMEU IN ENVIRONMENTAL STUDIES: Release of phosphate from lake sediment under different sulphate concentration 1/2 Nitrogen for anaerobic conditions Sample preparation in lab Long incubation in + 4 o C

15 PMEU IN ENVIRONMENTAL STUDIES: Release of phosphate from lake sediment under different sulphate concentration 2/ PO 4 -P -pitoisuus 1. kokeessa PO 4 -P µg/l SO 4 7,3 µg/l 31 µg/l µg/l Heitto, L., Heitto, A., Torssonen, J., Hakalehto, E. (2015). Effect of sulphate concentration on phosphate mobilization from lake sediment - an experimental study. Poster Presentation in 21 st International Conference on Environmental Indicators (ICEI 2015). Windsor, Canada. Journal of Environmental Indicators, 9:

16 Fast detection of Mycobacterium sp. Hakalehto, E. Pathophysiology

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18 a) b) The influence on growth of Enterobacter cloacae in the PMEU Spectrion in TYG medium in presence of different antibiotics. a- E. cloacae in TYG medium (control), b with penicillin G, c with cefuroxime, d with netilmicin. c) d) Hakalehto, E Antibiotic resistance traits of facultative Enterobacter cloacae strain studied with the PMEU (Portable Microbe Enrichment Unit). In: Antonio Méndez-Vilas (ed.) Science against microbial pathogens: communicating current research and technological advances, Formatex Research Center, Badajoz. Spain: Microbiology Series N:o3. Vol. 2. pp

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20 Samgina, T. Yu et al Journal of Proteome Research American Chemical Society Figure 4. (a) Growth of Staphylococcus aureus strain in the TYG medium without peptide additions; (b) growth of Staphylococcus aureus strain in the presence of 0.01 mg/ml of M3 sample; (c) growth of Staphylococcus aureus strain in the presence of 0.03 mg/ml of M3 sample.

21 Hakalehto, E., Enhanced microbial process in the sustainable fuel production. In: Boehm, R.F., Yang, H., Yan, J. (Eds.), Handbook of clean energy systems. John Wiley & Sons, Inc, Chichester, West Sussex, UK.

22 VISIONS OF JULES VERNE 1/2 "And what will they burn instead of coal?" "Water," replied Harding. "Water!" cried Pencroft, "water as fuel for steamers and engines! Water to heat water! Yes, my friends, I believe that water will one day be employed as fuel, that hydrogen and oxygen which constitute it, used singly or together, will furnish an inexhaustible source of heat and light, of an intensity of which coal is not capable... The Mysterious Island (LÍle Mysteriéuse 1874), by Jules Verne

23 VISIONS OF JULES VERNE 2/2...Some day the coalrooms of steamers and the tenders of locomotives will, instead of coal, be stored with these two condensed gases, which will burn in the furnaces with enormous calorific power. There is, therefore, nothing to fear. As long as the earth is inhabited it will supply the wants of its inhabitants,and there will be no want of either light or heat as long as the productions of the vegetable, mineral or animal kingdoms do not fail us. I believe, then, that when the deposits of coal are exhausted we shall heat and warm ourselves with water. Water will be the coal of the future." The Mysterious Island (LÍle Mysteriéuse 1874), by Jules Verne

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25 FATE OF CARBON IN INDUSTRIAL SYSTEMS PAST IN PAPER AND PULP PRODUCTION CO 2 WOOD WASTE CELLULOSE PAPER COMBUSTION WASTE PAPER WASTE WATER Hakalehto, E. (2015). Gas detection as control means of microbial metabolism in biorefineries and for the reduction of environmental emissions. Oral presentation in 21 st International Conference on Environmental Indicators (ICEI 2015). Windsor, Canada. Journal of Environmental Indicators, 9: SLUDGE

26 FATE OF CARBON IN INDUSTRIAL SYSTEMS FUTURE IN FOREST BIOREFINERIES WASTE PAPER WOOD WASTE SIDE STREAM CHEMICALS CELLULOSE PAPER COMBUSTION RECYCLED WATER WASTE WATER H 2 CH 4 BIOPROCESSES ENERGY, FUEL, BIOCHEMICALS ORGANIC FERTILIZERS CO 2 TO ASSIMILATION Adj. Prof. Elias Hakalehto, ICEI, Windsor, Canada, SLUDGE

27 Kiitos!